Hi there,
kefir is probiotic culture with up to 30 different microbes in it, around 80-90% are bacteria and 10-20% yeast. Once you have yeast species as part of your culture you need to introduce air=oxygen so they can properly multiply and grow/function. The oxygen role is quite complex and it's importance differs between various yeast species, more info for example about Saccharomyces cerevisiae requirements can be found here
http://morebeer.com/articles/how_yeast_use_oxygenI ferment my kefir grains without “continuous” access of air (so tightly closed vessel), however I leave around 20-30% of the volume of my fermentation vessel empty which means filled with air. I’m likely to open it once per day or two, specifically to make the yeast happy supplying it at least with a bit of oxygen. It works for me long term, the grains grow well and produce nice and tasty ferment.
I’ve made a small experiment about growing kefir grains and posted some links to the kefir related knowledge here
http://kvasirdiary.wordpress.com/2012/06/26/increase-in-biomass-of-the-kefir-grains-during-their-growth/Best of luck with your experiments,
Sincerely,
FAA